Core Application Scenarios
GDF-8 lub hom phiaj cov yam ntxwv ua rau nws muaj txiaj ntsig zoo hauv ntau qhov kev tshawb fawb txog kev ua haujlwm:
* ** Cov Kab Mob Metabolic thiab Cov Muscle Atrophy Kev Tshawb Fawb: ** GDF-8 qhia feem ntau yog nce siab hauv cov qauv ntawm cov kab mob xws li Duchenne leeg nqaij dystrophy thiab mob qog noj ntshav cachexia.
Inhibiting GDF-8 kev ua los ntawm noob caj noob ces (CRISPR/Cas9), RNA cuam tshuam (siRNA), los yog monoclonal antibodies (xws li Emugrobart) yog txoj hauv kev tshawb fawb tseem ceeb rau kev ncua cov leeg nqaij, txhim kho insulin tsis kam, thiab hais txog kev rog rog ntsig txog metabolic abnormality.
* ** Kev Tshawb Fawb Txog Kab Mob plawv: ** GDF-8 tsis yog tsuas yog cov leeg nqaij xwb tab sis kuj yog biomarker rau kab mob plawv. GDF-8 qib tau nce siab hauv cov neeg mob uas mob myocardial infarction (AMI) thiab lub plawv tsis ua haujlwm, thiab muaj kev cuam tshuam zoo nrog cov cim kev raug mob myocardial (xws li peak troponin I qib). Nws feem ntau yog siv los ua qhov taw qhia tseem ceeb rau kev ntsuas qhov hnyav ntawm myocardial infarction thiab kev loj hlob ntawm myocardial fibrosis.
* ** Cov Tshuaj Tiv Thaiv Kev Tiv Thaiv Kev Tiv Thaiv thiab Kev Tiv Thaiv Kev Tiv Thaiv:** Kev txiav tsis ntev los no - kev tshawb fawb ntug tau pom tias GDF-8 kuj tau hais tawm hauv cov poj niam cev xeeb tub. Thaum lub sij hawm tswj ovulation induction nyob rau hauv vitro fertilization (IVF), ntshav GDF-8 qib qhia dynamic hloov, thiab lawv cov concentration yog ze ze rau cov qib progesterone thiab soj ntsuam cev xeeb tub tus nqi, muab ib tug tshiab endocrinological foundations rau kwv yees IVF cov txiaj ntsig.
Kev tshawb fawb Reagent Siv Cov Lus Qhia Hauv vitro kev tshawb fawb ntawm GDF-8 feem ntau koom nrog cov proteins sib txuas, tshuaj tiv thaiv kab mob, thiab cov khoom siv tshawb nrhiav. Cov txheej txheem tshwj xeeb yuav tsum nruj me ntsis ua raws li cov lus qhia hauv qab no:
Reconstitution thiab cia ntawm Recombinant Proteins: Commercially available recombinant GDF-8 proteins feem ntau yog lyophilized hmoov. Ua ntej siv, lawv yuav tsum tau luv luv centrifuged kom cov hmoov nyob rau hauv qab ntawm lub raj. Nws raug nquahu kom siv cov dej tsis huv los yog cov chaw tsim khoom tau teev tseg rau kev rov tsim kho dua tshiab, nrog rau qhov concentration feem ntau tsis qis dua 0.1 mg / mL. Reconstituted proteins yog heev raug rau inactivation thiab yuav tsum tau aliquoted raws li ib zaug xwb- sim dosages thiab khaws cia nyob rau hauv ib tug ultra- kub freezer ntawm -20℃los yog -80℃. Rov ua dua lub voj voog khov-thaw yog txwv nruj.
ELISA Quantitative Detection: Thaum txiav txim siab qhov concentration ntawm GDF-8 hauv cov ntshav, ntshav, lossis cell kab lis kev cai supernatant, tshwj xeeb yuav tsum tau them rau qhov kev kho "ua kom" ntawm tus qauv. Vim hais tias circulating GDF-8 feem ntau tshwm sim nyob rau hauv ib tug latent complex daim ntawv, ncaj qha siv nrog cov pa ELISA tej zaum yuav ua rau tsis muaj cuav. Qee qhov kev kuaj pom muaj txiaj ntsig siab (xws li Ella microfluidic system) xav tau cov kua qaub ua kom cov qauv hloov latent GDF-8 mus rau hauv lub xeev tsis muaj zog tiv thaiv kab mob, ua raws li kev ua kom tsis zoo nrog alkali mus rau lub cev pH (7.2-7.6) ua ntej kuaj pom.
Cellular Function Validation: koob tshuaj- nyob ntawm qhov kev soj ntsuam kev loj hlob ntawm kev tiv thaiv kab mob feem ntau yog siv los ntsuas cov bioactivity ntawm recombinant GDF-8. Piv txwv li, MPC-11 myoblast cell kab yog siv los txiav txim qhov ib nrab-qhov siab tshaj plaws koob tshuaj (ED50, feem ntau nyob rau hauv thaj tsam ntawm 10-40 ng / mL) los ntawm kev soj ntsuam cov nyhuv inhibitory ntawm GDF-8 ntawm cell proliferation.
Kev cia khoom
Lyophilized hmoov (tsis qhib): kaw thiab tiv thaiv los ntawm lub teeb, 2-8℃rau luv luv -lub sij hawm ua hauj lwm cia (lub lis piam mus rau lub hlis), -20℃rau standard long-term cia (12-24 lub hlis), -80℃rau txuas ntxiv txee lub neej; thauj ntawm xiav dej khov.
Reconstituted solution: Khw muag khoom rau 7-30 hnub ntawm 2-8℃(7 hnub rau kev soj ntsuam, txuas ntxiv mus rau 30 hnub rau kev sim kab lis kev cai niaj hnub); tsis txhob khov-thaw-disulfide dimers yog qhov rhiab heev rau khov-thaw denaturation, yuav tsum tau aliquoted rau kev siv ib zaug.
Ntev -lub sij hawm aliquoted tov: Muaj 0.1% BSA los yog 5% trehalose cab kuj, concentration Ntau tshaj los yog sib npaug rau 10 µg/mL, aliquot ntim Ntau tshaj los yog sib npaug rau 10 µL los tiv thaiv adsorption, -20℃rau 3-6 lub hlis, -80℃rau 12 lub hlis; zam qhov kub thiab txias dua.
Khaws kom tsaus nti, siv cov -cov pa nqus dej qis, thiab tsis txhob vortex thaum rov tsim kho.
FAQ
Q: Qhov kev txiav txim yam tsawg kawg nkaus yog dab tsi?
A: Yam tsawg kawg nkaus kom muaj nuj nqis yog 10 fwj / teeb.
Q: Nws siv sijhawm ntev npaum li cas rau nkoj?
A: Hauv - cov khoom xaj yuav raug xa tuaj hauv 24 teev.
Q: Dab tsi purity koj tuaj yeem muab tau?
A: Txheem purity 99%.
Q: Qhov kev pabcuam courier koj siv?
A: Koj tuaj yeem xaiv FedEx, DHL, UPS, lossis kev pabcuam xa ntawv.
Q: Ntev npaum li cas shipping?
A: Kwv yees li 10-15 hnub.
Q: Koj puas tuaj yeem muab COA (Certificate of Account) daim ntawv tshaj tawm kev soj ntsuam zoo thiab HPLC (High-Performance Computing) qhia?
A: Yog lawm, tag nrho cov khoom raug kuaj xyuas ua ntej ua zaub mov xa tuaj.
Q: Koj puas txhawb OEM hom?
A: Peb txhawb OEM branding cov kev pab cuam, raws li kev txiav txim yam tsawg kawg nkaus kom muaj nuj nqis.
Q: Dab tsi yog koj tom qab - cov ntsiab lus ntawm kev muag khoom?
A: Tom qab tus neeg siv khoom tau txais cov khoom, yog tias qhov teeb meem zoo tau lees paub los ntawm peb cov khoom, lawv tuaj yeem txaus siab rau kev hloov pauv dawb lossis nyiaj rov qab. Peb ua raws li lub hauv paus ntsiab lus ntawm kev ua haujlwm thiab lub luag haujlwm, ua tib zoo teb thiab daws txhua yam teeb meem, yam tsis muaj lub luag haujlwm lossis zam teeb meem, thiab tiv thaiv cov neeg siv khoom muaj cai.
Q: Txoj kev them nyiaj twg muaj?
A: Peb txhawb nqa kev hloov pauv hauv txhab nyiaj, Bitcoin, Hloov pauv, thiab Xoom.
Cim npe nrov: sab saum toj- qib kev tshawb fawb tshawb fawb khov-qhuav hmoov gdf-8 1mg/vial cas: 271597-12-7, Tuam Tshoj sab saum toj-qib kev tshawb fawb kev tshawb fawb khov-dried hmoov gdf-8 1mg/vial cas: 271597, manufacturers, hoobkas
