Daim ntawv thov Scenario
Muscle Biology Research: Tshawb xyuas cov nyhuv inhibitory ntawm GDF-8 ntawm myoblast proliferation thiab sib txawv, tshawb xyuas cov txheej txheem molecular ntawm cov kab mob xws li cov leeg nqaij atrophy thiab cov leeg nqaij dystrophy.
Kev Tshawb Fawb Txog Kab Mob Metabolic: Tshawb xyuas lub luag haujlwm ntawm GDF-8 hauv lipid thiab qabzib metabolism, thiab nws koom nrog kev rog thiab metabolic syndrome.
Kev Tshuaj Ntsuam Xyuas thiab Kev Txhim Kho Tshuaj: Raws li lub hom phiaj molecule, siv los tshuaj xyuas lossis ntsuas cov tshuaj tiv thaiv kab mob, tshuaj tiv thaiv kab mob, lossis cov tshuaj sib xyaw me me uas tsom mus rau GDF-8 / myosin txoj hauv kev.
Stem Cell Research: Siv los kawm txog kev tswj xyuas cov teebmeem ntawm GDF-8 ntawm qhov sib txawv ntawm myogenic ntawm mesenchymal qia hlwb, thiab nws lub luag haujlwm hauv qia cell microenvironment.
Regenerative Medicine: Siv rau hauv cov leeg nqaij engineering thiab kev tshawb fawb ntawm tes, tshawb nrhiav cov tswv yim los txhawb cov leeg nqaij thiab kho.
Cia thiab siv txoj kev
Kev khaws cia thiab kev tuav pov hwm yog qhov tseem ceeb rau kev tswj hwm bioactivity ntawm GDF-8 protein. Thov ua raws li cov lus qhia nram qab no:
1. Daim foos tshuaj thiab lub xeev
Cov khoom no yog lyophilized hmoov lim los ntawm 0.2 μm lim nyob rau hauv sterile tej yam kev mob.
2. Kev cia khoom
Lyophilized Powder: Pom zoo rau ntev - lub sijhawm cia ntawm -20℃txog -80 degree, nws tuaj yeem ruaj khov rau 2 xyoos. Nws tuaj yeem khaws cia rau lub sijhawm luv luv ntawm chav sov li 3 lub lis piam.
Reconstituted Solution: Nyob rau hauv ib qho kev daws teeb meem uas tsis muaj cov khoom thauj (xws li 0.1% BSA, 5% HSA, 10% FBS, lossis 5% trehalose), nws tuaj yeem khaws cia ntawm 2-8℃rau 2-7 hnub. Rau kev khaws cia ntev dua, nws raug nquahu kom aliquot thiab khov ntawm -20℃rau -80 degree, qhov twg nws tuaj yeem ruaj khov rau 3-6 lub hlis.
3. Cov txheej txheem kho dua tshiab (theem tseem ceeb)
Centrifugation: Ua ntej qhib, centrifuge cov raj lyophilized nyob rau hauv lub siab -ceev centrifuge (kwv yees li 10,000 rpm los yog 12,000 rpm rau 30 vib nas this, los yog 3,000-3,500 rpm rau 5 feeb) kom tso cai rau cov phab ntsa hauv qab los yog lyophil. txo cov hmoov poob.
Aseptic Dissolution: Redissolve siv sterile deionized dej (ddH₂O). Kev daws teeb meem tsawg kawg yog 100 ug / mL raug pom zoo. Tom qab ntxiv dej, tsis txhob vortexing los yog muaj zog tshuab. Hloov chaw, siv maj mam co, qeeb inversion, lossis qis- nrawm pob zeb los pab kev sib cais thiab tiv thaiv cov protein denaturation.
Dilution thiab Aliquoting: Tom qab kev sib tov meej, dilute mus rau qhov ua hauj lwm concentration (pom zoo tsawg kawg yog 10 ug / mL) nrog ib tug tsis muaj protein ntau carrier, raws li cov kev sim tom ntej. Aliquot nyob rau hauv ib zaug -siv kom muaj nuj nqis, tsis txhob rov khov -thaw cycles. Aliquot ntim yuav tsum yog tsawg kawg yog 10 μL.
Vim li cas thiaj xaiv peb?
Hauv kev yuav khoom ntawm cov ntaub ntawv tshawb fawb tseem ceeb, xaiv ib tus neeg muag khoom ntseeg tau yog qhov tseem ceeb los ua kom ntseeg tau qhov tseeb ntawm cov ntaub ntawv sim thiab kev ua tiav ntawm cov haujlwm. Raws li cov kws tshaj lij cov chaw tsim khoom, peb muaj cov hauv qab no irreplaceable zoo:
Qhov Chaw Tsim Khoom, Tus Nqi Tswjhwm: Peb yog R&D ncaj qha thiab tsim cov hauv paus, tshem tawm cov kev sib txuas nruab nrab thiab muab cov nqi sib tw rau koj, tshwj xeeb tshaj yog tsim nyog rau lub sijhawm ntev -, loj - ntim khoom xav tau.
Cov cuab yeej tshaj lij, Kev Siv Tshuab Ua Ntej: Peb muaj cov khoom lag luam paub tab thiab qib siab tshaj plaws thiab ua kom huv si platform (nrog rau cov tshuab prokaryotic thiab eukaryotic), nruab nrog cov cuab yeej tshaj lij los xyuas kom meej thiab tswj cov khoom lag luam rau txhua pawg ntawm cov khoom.
Kev Ruaj Ntseg, Muaj Peev Xwm Ntau Lawm: Raws li tus neeg xa khoom qub, peb muaj peev xwm loj -, ua kom muaj kev ruaj khov thiab ruaj khov ntawm cov khoom, tshem tawm koj cov kev txhawj xeeb txog kev xa khoom mus sij hawm thaum koj sim.
High Purity, Superior Quality: Peb cov khoom tau dhau los ua cov txheej txheem ua kom huv huv, nrog kev huv huv ntau dua 95% raws li tau lees paub los ntawm SDS-PAGE. Qib Endotoxin, raws li kev kuaj pom los ntawm LAL txoj kev, yog <1 EU / ug, ua kom muaj kev ua haujlwm lom neeg siab (xws li, ED₅₀ tus nqi txiav txim los ntawm MPC-11 cell proliferation inhibition assay).
Tom qab-Kev Pabcuam Muag Khoom, Kev txhawj xeeb-Kev Pabcuam Dawb: Peb muab kev pabcuam kev tshaj lij thiab kev pabcuam tomqab-muag kev pabcuam, cog lus rau cov khoom lag luam zoo, thiab muab tag nrho- txheej txheem kev txhawb nqa los ntawm kev sib tham mus rau daim ntawv thov.
FAQ
Q: Koj cov khoom tsim nyob qhov twg?
A: Peb cov khoom yog los ntawm Shenzhen, Suav.
Q: Qhov kev txiav txim yam tsawg kawg nkaus yog dab tsi? A: Yam tsawg kawg nkaus kom muaj nuj nqis yog 10 fwj / teeb.
Q: Nws siv sijhawm ntev npaum li cas rau nkoj?
A: Hauv - cov khoom xaj yuav raug xa tuaj hauv 24 teev.
Q: Dab tsi purity theem koj tuaj yeem muab tau?
A: Txheem purity 99%.
Q: Qhov kev pabcuam courier koj siv?
A: FedEx, DHL, UPS, thiab kev pabcuam xa ntawv muaj.
Q: Ntev npaum li cas shipping?
A: Kwv yees li 10-15 hnub.
Q: Koj puas tuaj yeem muab COA (Certificate of Analysis) daim ntawv qhia thiab HPLC (High-Performance Computing) qhia?
A: Yog lawm, tag nrho cov khoom raug kuaj xyuas ua ntej ua zaub mov xa tuaj.
Q: Koj puas txhawb OEM / ODM?
A: Kev pabcuam OEM / ODM tau txais kev txhawb nqa, ua raws li qhov yuav tsum tau ua kom tsawg kawg nkaus.
Q: Dab tsi yog koj tom qab - cov ntsiab lus ntawm kev muag khoom?
A: Tom qab tus neeg siv khoom tau txais cov khoom, yog tias qhov teeb meem zoo tau lees paub los ntawm peb cov khoom, lawv tuaj yeem txaus siab rau kev hloov pauv dawb lossis nyiaj rov qab. Peb ua raws li lub hauv paus ntsiab lus ntawm kev ua haujlwm thiab lub luag haujlwm, ua tib zoo teb thiab daws txhua yam teeb meem, yam tsis muaj lub luag haujlwm lossis zam teeb meem, thiab tiv thaiv cov neeg siv khoom muaj cai.
Q: Txoj kev them nyiaj twg muaj?
A: Peb txhawb kev hloov txhab nyiaj hauv txhab nyiaj, Bitcoin, Transferwise, thiab Xoom.
Cim npe nrov: gdf-8 1mg/vial yog siv rau kev tshuaj ntsuam tshuaj antibody tshuaj thiab tshawb fawb kab mob metabolic., Tuam Tshoj gdf-8 1mg/vial yog siv rau kev tshuaj ntsuam tshuaj antibody thiab tshawb fawb kab mob metabolic. manufacturers, lwm tus neeg, Hoobkas
