Humanin yog 24-amino acid endogenous teeb liab peptide encoded los ntawm mitochondrial DNA, teej tug mus rau mitochondrial-derived peptide (MDP) tsev neeg. Nws tau pib pom nyob rau hauv kev sim tshuaj ntsuam rau Alzheimer's kab mob neuronal ciaj sia taus. Nws thim rov qab cov kev txawj ntse, ua kom pom tias mitochondria, ntxiv rau kev nkag mus rau cov kab mob ua pa, tuaj yeem tsim cov peptides bioactive muaj peev xwm ntawm kev sib txuas lus hauv nruab nrog cev thiab extracellular. Raws li cov khoom siv raw rau khov-kev tshawb fawb peptide qhuav, nws tsis txwv rau ib lub hom phiaj ntawm cov ntaub so ntswg, tab sis tuaj yeem tiv thaiv cov kab mob intracellular mitochondria thiab pab txhawb kev hloov pauv ntawm cellular receptor teeb liab. Nws cov theem endogenous qhia maj mam poob nrog lub hnub nyoog thiab muaj feem cuam tshuam nrog lub hnub nyoog ntsig txog cellular vulnerability.
Humanin qhov txiaj ntsig zoo tshaj plaws yog nyob rau hauv nws ntau yam, dav -spectrum tiv thaiv cov teebmeem. Kev tshawb fawb thaum ntxov ua rau nws muaj koob meej rau nws lub peev xwm los tiv thaiv lub hlwb los ntawm Alzheimer's -txog neurotoxicity, tab sis tom qab kev tshawb fawb tshawb fawb tau pom tias nws txoj kev nthuav dav dhau qhov no. Nws tsis tsuas yog txhim kho tag nrho cov tshuaj insulin rhiab heev thiab koom nrog hauv kev tswj hwm ntawm cov piam thaj metabolism, tab sis kuj tseem ua lub luag haujlwm tseem ceeb hauv kev tswj hwm cov hlab ntsha, tiv thaiv kev puas tsuaj ischemic, thiab txawm tias qeeb tag nrho cov txheej txheem kev laus. Raws li cov kws tshawb fawb tau tshawb pom tias nws yog ib tsev neeg loj ntawm "mitochondrial- tau muab los ntawm peptides," lub luag haujlwm tseem ceeb ntawm humanin raws li tus qauv qauv hauv kev noj qab haus huv metabolic thiab txoj hauv kev ua neej ntev tau ua kom pom tseeb.
Lub Hom Phiaj Shortcomings
Muaj ob lub methionines, Met¹ thiab Met²⁰, ntxiv rau Cys⁸ dawb sulfhydryl pawg, nws cov haujlwm tau txo qis vim yog ob txoj hauv kev degradation tseem ceeb hauv aqueous theem oxidation (Met → MetO) thiab disulfide dimerization. Kev tsis huv ntawm lub cev tsis zoo ua rau muaj qhov tsis zoo ntawm kev ua haujlwm, uas yog nws qhov tseem ceeb tshaj plaws ntawm lub cev tsis muaj zog.
Kev nkag mus rau BBB tsis zoo ua rau muaj lus nug txog seb puas muaj kev tswj xyuas cov khoom siv peripheral tuaj yeem ua tiav cov txiaj ntsig zoo hauv lub hauv paus paj hlwb. Intranasal thiab intraventricular tus me nyuam tsuas yog tso tawm cov ntaub ntawv zoo siab hauv PD / AD tsiaj; subcutaneous/peritoneal tus me nyuam yog raws li tib neeg lub tswv yim, tsis pov thawj.
Tag nrho cov ntaub ntawv nyuaj yog txwv rau cov ntaub ntawv xov tooj ntawm tes thiab nas; Hauv tib neeg, tsuas yog cov ntaub ntawv soj ntsuam muaj nyob (plasma qib qis hauv cov kab mob plawv / AD cov neeg mob, qib siab hauv centenarians). Muaj pes tsawg RCTs rau kev kho mob siv humanin, uas tsis tau lees paub los ntawm FDA / EMA thiab poob rau hauv RUO (Reactive Organic Drug) qeb.
Kev tiv thaiv -apoptotic mechanism yog ob- ntaj ntaj: Bax inhibition + STAT3 ua kom pom tseeb yog qhov muaj peev xwm ua tau nyob rau hauv cov kab mob qog noj ntshav. Txawm hais tias tus tsiaj chemotherapy qauv qhia tias tsis muaj kev tiv thaiv qog, tawm ntawm - cov teebmeem hauv tib neeg cov qog nqaij hlav tau raug txiav tawm.
Kev tshawb fawb- qib kev lag luam muaj cov qauv tsim feem ntau yog HNG, nrog cov tsiaj qus- hom HN tsis tshua muaj. Ib lub raj mis uas sau tias "Humanin 10mg" yeej muaj HNG; kev twv ua ntej yuav tsum tau kuaj xyuas COA ua ntej txiav txim siab.
Kev cia khoom:
Cov khoom tiav lawm feem ntau yog sterile lyophilized peptide hmoov. Cov saw peptide yog rhiab heev rau oxidation, av noo, thiab rov qab kub hloov. Oxidative kev hloov kho yuav rhuav tshem nws lub peev xwm los khi rau Bax thiab nws lub peev xwm paub txog receptor, ncaj qha ua rau poob ntawm kev ua haujlwm lom neeg.
Unopened lyophilized hmoov: kaw, tiv thaiv los ntawm lub teeb, thiab khaws cia qhuav; cia ntawm -20℃. Nws raug nquahu kom siv tsis pub dhau 12 lub hlis. Xwb, khaws cia ntawm -80 degree, kaw, thiab tiv thaiv lub teeb; Lub neej txee tuaj yeem ncav cuag 24 lub hlis. Rov ua dua lub voj voog khov-thaw yog txwv nruj.
Tom qab rov tsim kho dua tshiab, cov qauv ua kua yuav tsum tau muab faib ua ib qho - siv feem, khaws cia ntawm -80℃hauv lub teeb- pov thawj, ntim ntim, thiab siv hauv 6 lub hlis; tsuas yog luv heev- lub sij hawm cia ib ntus tso cai ntawm -20℃; Lub sij hawm ntev cia ntawm 4℃yog txwv tsis pub, thiab cia nyob rau hauv chav tsev kub yog nruj me ntsis txwv tsis pub.
Kev qhib kev ua haujlwm yuav tsum tau ua sai sai hauv qhov qis - qhov kub thiab txias ib puag ncig kom txo cov hmoov lub sij hawm sib cuag nrog huab cua, noo noo, thiab oxygen; kom deb ntawm ultraviolet lub teeb thiab muaj zog oxidizing tus neeg sawv cev.
Cov kev ceev faj
Daim ntawv RUO txhais tau tias "Tsis Siv Neeg." Cov tshuaj subcutaneous / qhov ntswg rau kev siv tus kheej ua txhaum cai; muas vials tsis sib npaug rau cov tshuaj.
Wild- hom HN cell sim yuav tsum tau ua ntawm 1–100 nM, thiab HNG ntawm 0.1–10 nM. Tsiaj intraperitoneal/nasal assays yuav tsum tau tsim nyob rau theem ug/kg; tsis ncaj qha muab cell concentration los ntawm lub cev hnyav.
COA yuav tsum suav nrog HPLC Ntau dua lossis sib npaug li 98%, MS txheeb xyuas, Met oxidation ncov piv, thiab endotoxin; rau HN daim ntawv lo tab sis qhov tseeb HNG, qhov no yuav tsum tau hais kom meej meej nyob rau hauv qhov ua ntu zus.
Ceev faj txog cov tshuaj stacking: Nws tuaj yeem cuam tshuam nrog kev txhais lus phenotypic thaum siv nrog STAT3 inhibitors (xws li cov tshuaj tiv thaiv zoo li qub), Bax probes, thiab IGF-1 systems. Ib qho kev tsis zoo yuav tsum raug suav nrog hauv pawg tswj hwm (xws li kev sib tw siv PNC-29 mus kom ze).
Tsis txhob thaum cev xeeb tub thiab lactation, thaum muaj cov qog nqaij hlav ua haujlwm, thaum lub sijhawm muaj tus kab mob autoimmune, lossis hauv cov neeg mob nkag siab rau cov khoom siv ntawm tes; broad -spectrum anti- kev ua apoptosis ua rau muaj kev pheej hmoo rov qab hauv cov xwm txheej no.
Kev txhaj tshuaj ntawm qhov chaw mob / mob taub hau tsis tshua muaj feem ntau yog tus kheej - tshaj tawm suab nrov hauv zej zog, tab sis tsis muaj kev tshawb fawb txog qib - qib kev nyab xeeb profile tau tsim rau tib neeg. Yog tias muaj kev tsis xis nyob tsis tu ncua, tsis txhob sim ua siab ntev.
Cim npe nrov: Hoobkas zoo tshaj -muag peptide humanin cas: 330936-69-1, Tuam Tshoj Hoobkas zoo tshaj plaws muag peptide humanin cas: 330936-69-1 manufacturers, lwm tus neeg, Hoobkas
